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Analyse over sample variants with Site Profiler

Site Profiler is a tool which allow you analyse your data over sample variants and compare between sites. This makes it possible to identify and monitor changes in your data over variants. With

  • Generate a Chart of sample values over variants. An example is shown below where the axis have been swapped to more intuitively display sample values over depth, however this is not the default chart. For more information about the types of charts that can be created with Site Profiler please see below.

  • Generate a Table containing the queried data

  1. Open Site Profiler, and select the pool you wish to access.

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  2. Select the parameter you wish to analyse.

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  3. Next select sites you wish to view data at.

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  4. A drop down menu should appear, prompting you to select samples. The sample list will contain the samples that have been taken at each of the locations, and from this menu you can select the samples you wish to view in your chart.

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  5. After this click, Get Data to generate a chart.

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  6. Obviously, this chart needs to be edited to make it more readable. First click Chart Presets. img

  7. Next we'll check Reverse Y-axis and Values X-axis, because the variant being analysed over is depth, this will make the chart more intuitive. Then click the Back Arrow in the top left of the Task Pane to return to the main page and select get data again to re-generate the chart with the new settings. img
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  8. The chart is still not very readable, the range of the x-axis needs to be changed to make the x-axis more readable. Click Chart Presets and then modify the X-axis Minimum and X-axis Maximum, and then hit the back arrow and re-generate the chart. img img

  9. To make further changes to the chart generated clickChart Presets, after changing the chart settings click the back arrow in the top left corner and then Get Data again to re-generate the chart.

  10. Once you've generated a chart you're happy with, change the name of the sheet its on to something other than SBK_Chart, this will stop it from being closed or altered by the Splashback Excel Add-in. This chart can be further edited using Excel's chart tools.